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Phone: +1 732 380 8900 In order to understand how buffer solutions maintain a constant pH, let us consider the example of a buffer solution containing sodium acetate and acetic acid. All rights reserved. Is this normal? © 2008-2020 ResearchGate GmbH. A solution which has a stable pH is termed as a buffer solution. To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. 2. Ammonium acetate is a salt, not a buffer! Other important uses of this compound include: 2) Is it necessary to prepare the buffer solution using ultra pure water or distilled water is tolerated? Prepare 800 mL of distilled water in a suitable container. 1) When preparing an ammonium acetate 5mM buffer solution with pH=3.3, which is better to use to adjust the pH? Ammonium acetate is sufficiently volatile for LC-MS use, but we are left with the 2.0

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